Development of a Biologically Active Guglielmi Detachable Coil for the Treatment of Cerebral Aneurysms. Part I: In Vitro Study

Schematic diagram of parallel plate-type flow chamber used for applying flow shear stress to endothelial cells cultured on PS surface. PS-coated slide glass on which endothelial cells (BAECs) were confluently cultured was mounted together with a silicone rubber gasket to a lower PMMA plate. Flow shear stress was imposed on BAECs by circulating 1% PBS maintained at 37°C in reservoir by using pump.

Phase-contrast micrograph of BAECs on PS surface patterned by ion implantation (original magnification, ×100; diameter of a circle, 100 μm).

A, Day 1, no endothelial proliferation was observed on either protein-coated only (area A) or ion-implanted protein-coated (area B) surface.

B, Day 5, areas A and B demonstrated uniform proliferation of the BAECs.

C, Day 5, Post-trypsin treatment at 2 hours. Note most of BAECs were strongly attached on the ion-implanted circular area. Almost no BAECs were observed on surface coated with collagen only.

Scanning electron micrograph of BAECs on platinum plate patterned by ion implantation. (Note morphologic deformity of cells owing to effect of trypsin and glutaraldehyde.)

A, Day 5, areas A and B demonstrated uniform proliferation of BAECs.

B and C, Note strong BAEC adhesion only on circular ion-implanted protein-coated areas (original magnification, ×100 [for B]; ×400 [for C]).

Sequential changes in number of endothelial cells exposed to flow shear stress: 30 min (P < .001), 45 min (P < .001), 60 min (P < .05), 90 min (P < .01), 150 min (P < .05), 180 min (P = .1488).