Article Figures & Data
Figures
- Fig 1.
Diagram of miRNA regulation of gene expression. mRNA indicates messenger RNA; ORF, open reading frame; Pol, polymerase; Pri-miRNA, primary microRNA; RISC, RNA-induced silencing complex.
- Fig 2.
Top 10 canonical pathways identified by IPA. NFAT indicates nuclear factor of activated T cells.
- Fig 3.
Validation of RNA-sequencing results by real-time polymerase chain reaction assays. The real-time polymerase chain reactions are indicated by blue bars, and RNA sequencing is indicated by red bars. LCA indicates left carotid artery; OPN, osteopontin; TIMP1, TIMP metalloproteinase inhibitor 1; TYR, tyrosinase; TACR1, tachykinin receptor 1; vWF, von Willebrand factor.
Tables
Mature miRNA Fold Change P Value FDR hsa-miR-1 0.48 4.62E-05 1.01E-03 hsa-miR-9-5p 0.43 4.80E-05 1.01E-03 hsa-miR-10a-5p 2.66 8.83E-09 4.66E-07 hsa-miR-10b-5pa 2.45 3.47E-07 1.47E-05 hsa-miR-21-5p 3.51 4.43E-11 4.67E-09 hsa-miR-34a-5p 2.34 1.15E-04 2.02E-03 hsa-miR-34c-5pb 3.79 1.28E-06 4.38E-05 hsa-miR-146a-5p 3.68 1.45E-06 4.38E-05 hsa-miR-146b-5pc 3.32 9.08E-11 6.39E-09 hsa-miR-204-5p 0.48 7.32E-06 1.93E-04 hsa-miR-223-3p 3.95 6.79E-12 1.43E-09 Canonical Biologic Pathway No. of Genes Up-Regulated Genes No. of Genes Down-Regulated Gene(s) Dendritic cell maturation 25 CD80, CD83, CD86, CD1C, COL10A1, COL1A1, COL1A2, FCGR1B, FCGR2A, HLA-A, HLA-DMA, HLA-DMB, HLA-DOB, HLA-DQA1, HLA-DRA, HLA-DRB1, HLA-DRB5, IL15, IL1B, IL1RL2, IL1RN, PIK3CG, PIK3R5, PLCB2, TREM2 3 CREB5, PLCB4, PLCL1 Role of NFAT in regulation of the immune response 22 BLNK, BTK, CD80, CD86, FCER1A, FCGR1B, FCGR2A, FOS, GNG2, HLA-DMA, HLA-DMB, HLA-DOB, HLA-DQA1, HLA-DRA, HLA-DRB1, HLA-DRB5, LCP2, LYN, PIK3CG, PIK3R5, PLCB2, SYK 2 PLCB4, GNAO1 Antigen-presentation pathway 12 CD74, HLA-A, HLA-DMA, HLA-DMB, HLA-DOB, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB2, HLA-DRA, HLA-DRB1, HLA-DRB5 0 NA Altered T-cell and B-cell signaling in rheumatoid arthritis 17 CCL21, CD80, CD86, HLA-DMA, HLA-DMB, HLA-DOB, HLA-DQA1, HLA-DRA, HLA-DRB1, HLA-DRB5, IL15, IL1B, IL1RN, SPP1, TLR1, TLR8, TNFSF13B 0 NA Atherosclerosis signaling 19 ALOX5, CCL2, CCR2, COL10A1, COL1A1, COL1A2, CXCR4, IL8, IL1B, IL1RN, LYZ, MSR1, PDGFB, PDGFC, PDGFD, PLA2G7, PLA2R1, SELE, VCAM1 1 RBP4 Note:—NA indicates not applicable; NFAT, nuclear factor of activated T cells.
