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Research ArticleHead & Neck
Open Access

Human Papillomavirus, p16, and Epidermal Growth Factor Receptor Biomarkers and CT Perfusion Values in Head and Neck Squamous Cell Carcinoma

N.L. Hoefling, J.B. McHugh, E. Light, B. Kumar, H. Walline, M. Prince, C. Bradford, T.E. Carey and S.K. Mukherji
American Journal of Neuroradiology May 2013, 34 (5) 1062-1066; DOI: https://doi.org/10.3174/ajnr.A3349
N.L. Hoefling
aFrom the Departments of Radiology (N.L.H.)
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J.B. McHugh
bPathology (J.B.M.)
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E. Light
fBiostatistics Core (E.L.), and the Head and Neck SPORE Team, University of Michigan Comprehensive Cancer Center, Ann Arbor, Michigan.
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B. Kumar
cOtolaryngology (B.K., H.W., M.P., C.B., T.E.C.)
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H. Walline
cOtolaryngology (B.K., H.W., M.P., C.B., T.E.C.)
dCancer Biology Graduate Program (H.W.)
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M. Prince
cOtolaryngology (B.K., H.W., M.P., C.B., T.E.C.)
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C. Bradford
cOtolaryngology (B.K., H.W., M.P., C.B., T.E.C.)
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T.E. Carey
cOtolaryngology (B.K., H.W., M.P., C.B., T.E.C.)
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S.K. Mukherji
eDepartments of Radiology, Otolaryngology, Radiation Oncology, and Oral Medicine (S.K.M.), University of Michigan, Ann Arbor, Michigan
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    Fig 1.

    Distribution of Wilcoxon scores for CP in pretreatment patients. We see significantly lower CP in pretreatment patient tumors that overexpress EGFR.

  • Fig 2.
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    Fig 2.

    Distribution of Wilcoxon scores for CP in pretreatment patients. There is a trend toward higher CP in tumors that overexpress p16 and are HPV-positive.

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    Fig 3.

    Distribution of Wilcoxon scores for CP in pretreatment versus palliative patients. Pretreatment patients have significantly lower CP scores versus palliative patients.

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    Fig 4.

    Distribution of Wilcoxon scores for BF in pretreatment versus palliative patients. We see that pretreatment patients have significantly higher BF versus palliative patients.

Tables

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    Table 1:

    CTP parameter mean (95% CI) overall in pretreatment and palliative groups combined

    CTP ParameterMean (95% CI)
    BF103.7 (73.6–133.7)
    BV11.8 (3.7–19.9)
    CP27.4 (19.4–35.4)
    MTT7.6 (6.4–8.8)
    • Note:—CI indicates confidence interval.

    • View popup
    Table 2:

    CTP parameter mean (95% CI) by marker status in pretreatment and palliative groups combined

    PositiveNegativeP Valuea
    HPV(n = 5)(n = 19)
        BF121.4 (56.8–186.1)101.6 (63.5–139.6).53
        BV8.2 (5.1–11.4)13.2 (2.4–24.0).97
        CP30.4 (9.8–51.1)27.3 (17.4–37.2).67
        MTT7.4 (3.3–11.6)7.8 (6.5–9.1).52
    EGFRn = 15n = 5
        BF108.7 (60.5–156.9)105.4 (42.5–168.2).66
        BV14.7 (0.8–28.6)7.4 (5.4–9.4).93
        CP27.1 (16.7–37.6)37.3 (5.4–69.2).79
        MTT7.0 (5.4–8.5)7.8 (3.9–11.7).22
    • Note:—CI indicates confidence interval.

    • ↵a P value derived from the Wilcoxon 2-sample test.

    • View popup
    Table 3:

    CTP parameter mean (95% CI) overall in the pretreatment group

    CTP ParameterMean (95% CI)
    BF124.2 (87.4, 160.9)
    BV8.4 (6.1, 10.7)
    CP19.9 (15.8, 24.0)
    MTT7.1 (5.7, 8.5)
    • Note:—CI indicates confidence interval.

    • View popup
    Table 4:

    CTP parameter mean (95% CI) by marker status in the pretreatment group

    CTP ParameterPositiveNegativeP Valuea
    HPVn = 4n = 13
        BF134.5 (55.2–213.8)126.3 (77.0–175.7).69
        BV8.2 (3.5, 12.8)8.9 (5.9–12.0).95
        CP23.5 (12.0–35.0)19.4 (14.1–24.6).14
        MTT7.3 (1.2–13.3)7.2 (5.6–8.8).95
    EGFRn = 10n = 4
        BF137.1 (70.2–204.0)108.8 (16.9–200.7).72
        BV8.7 (4.4–13.1)7.0 (4.4–9.7).94
        CP16.2 (11.8–20.7)26.7 (8.9–44.4).04
        MTT5.8 (4.3–7.3)7.9 (2.1–13.7).44
    • Note:—CI indicates confidence interval.

    • ↵a P value derived from the Wilcoxon 2-sample test.

    • View popup
    Table 5:

    CTP parameter mean (95% CI) by pretreatment-versus-palliative status

    Pretreatment (n = 18)Palliative (n = 7)P Valuea
    BF124.2 (87.4–160.9)50.9 (19.1–82.7).01
    BV8.4 (6.1–10.7)20.4 (−13.6–54.5).86
    CP19.9 (15.8–24.0)46.5 (21.8–71.2).01
    MTT7.1 (5.7–8.5)9.0 (6.8–11.3).15
    • ↵a P value derived from the Wilcoxon 2-sample test.

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American Journal of Neuroradiology: 34 (5)
American Journal of Neuroradiology
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Cite this article
N.L. Hoefling, J.B. McHugh, E. Light, B. Kumar, H. Walline, M. Prince, C. Bradford, T.E. Carey, S.K. Mukherji
Human Papillomavirus, p16, and Epidermal Growth Factor Receptor Biomarkers and CT Perfusion Values in Head and Neck Squamous Cell Carcinoma
American Journal of Neuroradiology May 2013, 34 (5) 1062-1066; DOI: 10.3174/ajnr.A3349

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Human Papillomavirus, p16, and Epidermal Growth Factor Receptor Biomarkers and CT Perfusion Values in Head and Neck Squamous Cell Carcinoma
N.L. Hoefling, J.B. McHugh, E. Light, B. Kumar, H. Walline, M. Prince, C. Bradford, T.E. Carey, S.K. Mukherji
American Journal of Neuroradiology May 2013, 34 (5) 1062-1066; DOI: 10.3174/ajnr.A3349
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