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62 Pathological, transcriptomic and prognostic assessment of lymphoid aggregates in patients with primary and metastatic melanoma
Lilit Karapetyan, Aofei Li, Danielle Vargas De Stefano, Ayah Al-Bzour, Hassan Abushukair, Andrew Knight, Sabrina Bruno, Cindy Sander, Hong Wang, Yian Chen, Jane Messina, Walter J Storkus, John M Kirkwood

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A72; DOI: 10.1136/jitc-2023-SITC2023.0062
63 Infiltration by myeloid cells is an independent prognostic biomarker in vulvar squamous cell carcinoma, associated to stem cell-like features of the tumor
Ziena Abdulrahman, Kim Kortekaas, Mariette van Poelgeest, Sjoerd Van der Burg

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A73; DOI: 10.1136/jitc-2023-SITC2023.0063
64 Augmenting low-plex multiplex imaging by leveraging the potential of same-slide H&E analysis
Becky Arbiv, Assaf Debby, Nethanel Asher, Guy Ben-Betzalel, Ron Elran, Pinchas Birnbaum, Shai Bookstein, Tal Dankovich, Lena Tsabari, Yuval Shachaf, Yoad Cohen, Amit Bart, Oscar Puig, Kenneth Bloom, Ronnie Shapira-Frommer, Iris Barshack, Ettai Markovits

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A74; DOI: 10.1136/jitc-2023-SITC2023.0064
65 Development and optimization of a broad hyperplex immunofluorescence assay for tumor immune microenvironment characterization
Racheli Ben Shimol, Angela Crabtree, Lauren Hamilton, Jaylen Rosemon, James Denegre, Marco Cassano, Carlo Bifulco, Brian Piening

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A75; DOI: 10.1136/jitc-2023-SITC2023.0065
66 Switchable antibodies for increased throughput in iterative immuno-fluorescent staining of cancer tissue using ChipCytometry^TM
Thore Boettke, Marcus Boettcher, Anke Brix

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A76; DOI: 10.1136/jitc-2023-SITC2023.0066
67 Evaluation of precision and robustness of a flexible multiplex immunofluoresce workflow for immune-profiling analysis
Yvette Cajigas, Cassandra Kysilovsky, Erin Nicola, Ruben Cardenes, Friedrich Preusser, Angela Vasaturo

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A77; DOI: 10.1136/jitc-2023-SITC2023.0067
69 A novel protease-free method for the co-detection of RNA and protein biomarkers using the RNAscope™ technology
Laetitia Chatelain, Anji Bei, Nancy George, Ge-Ah Kim, Sonali Deshpande, Steve Zhou, Li-Chong Wang, Maithreyan Srinivasan

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A79; DOI: 10.1136/jitc-2023-SITC2023.0069
8 Antitumor and metabolomic evaluation of immune checkpoint inhibition in diet-induced obese mice
Paul Gonzales, Lara Reid

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A8; DOI: 10.1136/jitc-2023-SITC2023.0008
70 Deciphering the relationship between lymphocytes and macrophages within the tumor micro-environment using a workflow combining RNA transcripts detection and multiplex IHC on Brightplex®
Aurelie Collignon, Alex Trinh, Maïté Chamourin, Marion Olive, Lucie Sudre, Anthony Lebret, Alboukadel Kassambara, Giovanni Bussoti, Jérôme Galon, Jacques Fieschi

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A80; DOI: 10.1136/jitc-2023-SITC2023.0070
71 Integration of RNA in situ hybridization and sequential immunofluorescence for same-slide fully automated multi-omics analysis of the tumor microenvironment
Alice Comberlato, Arec Manoukian, Paula Juricic, Pino Bordignon, Alexandre Kehren, Alix Faillétaz, Anushka Dikshit, Emerald Doolittle, Rose Delvillar, Steve Zhou, Ching-Wei Chang, Li-Chong Wang

Journal for ImmunoTherapy of Cancer Nov 2023, 11 (Suppl 1) A81; DOI: 10.1136/jitc-2023-SITC2023.0071

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